Reactive oxygen species stimulate prostaglandin F2 production in human endometrial stromal cells in vitro

Reactive oxygen species stimulate prostaglandin F2 production in human endometrial stromal cells in vitro

Norihiro Sugino,1, Ayako Karube-Harada, Shiro Kashida, Shuji Takiguchi and Hiroshi Kato
Department of Obstetrics and Gynecology, Yamaguchi University School of Medicine, Minamikogushi 1–1–1, Ube 755-8505, Japan

BACKGROUND: The present study was undertaken to investigate the effect of reactive oxygen species on prostaglandin F2 (PGF2) production by human endometrial stromal cells (ESC). METHODS AND RESULTS: Isolated ESC were incubated with hydrogen peroxide, which induces lipid peroxidation. Hydrogen peroxide increased both intracellular and medium concentrations of PGF2 (P < 0.01). A time course study showed that hydrogen peroxide significantly increased PGF2 concentrations in the medium after 6 h incubation (P < 0.01), after which no further increase was observed. To study whether the increase in PGF2 production caused by hydrogen peroxide was mediated by cyclooxygenase, ESC were incubated with indomethacin (0.5 µg/ml), an inhibitor of cyclooxygenase, in the presence of hydrogen peroxide. Indomethacin significantly blocked the increases in PGF2 production caused by hydrogen peroxide (P < 0.01). Hydrogen peroxide also increased PGF2 production by decidualized ESC (P < 0.01), induced by the incubation with medroxyprogesterone acetate (10–6 mol/l) and oestradiol (10–8 mol/l). CONCLUSIONS: Reactive oxygen species stimulate PGF2 production in ESC, suggesting that they might influence endometrial function by regulating PGF2 production.


Key words: endometrial stromal cell/human/hydrogen peroxide/prostaglandin F2/reactive oxygen species

1 To whom correspondence should be addressed. E-mail: obgyn@po.cc.yamaguchi-u.ac.jp

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